Población de proteoglicanos en diferentes sitios anatómicos de la cartílaga articular de la rodilla bovina

Autores/as

  • Marcelo Augusto Marretto Esquisatto Centro Universitário da Fundação Hermínio Ometto FHO https://orcid.org/0000-0001-9786-6107
  • Renata Erbert Contriciani Centro Universitário da Fundação Hermínio Ometto FHO
  • Edson Rosa Pimentel Centro Universitário da Fundação Hermínio Ometto FHO
  • Laurecir Gomes Centro Universitário da Fundação Hermínio Ometto FHO

DOI:

https://doi.org/10.24933/rep.v7i1.301

Palabras clave:

Forças biomecânicas, glicosaminoglicanos, variação anatômica

Resumen

Las fuerzas mecánicas que actúan sobre una articulación sinovial inducen cambios en la composición y organización del cartílago articular. Los condrocitos responden a los estímulos tanto de manera cuantitativa como cualitativa en relación a la matriz extracelular. En el presente estudio, se investigaron cinco regiones diferentes de la articulación de rodilla bovina en términos de poblaciones de proteoglicanos y su contenido de glucosaminoglicano. La población de PG de alta densidad fue aislada por ultracentrifugación (fracción D1) y filtración en gel. El PG presentó tamaño similar en todas las regiones, pero en la tibia estas moléculas presentan mayor Mr. El sulfato de condroitina fue el glucosaminoglicano más prominente detectado en la fracción D1 y sus cadenas presentaron alrededor de 40 KDa. Dos poblaciones de PG no agregantes se aislaron en cantidades similares de todas las regiones. Estas moléculas mostraron características polidispersas y migrando alrededor de 70 y 200 kDa y mostrando un comportamiento similar al de los pequeños proteoglicanos decorin y biglican. La presencia de decorin fue confirmada por immunoblotting. El análisis del gel de agarosa después de la digestión con papaína mostró la presencia de dermatan sulfato en estos PGs. La presencia de decorin y en particular de biglicano en la fracción D2 sugiere que estos pequeños proteoglicanos pueden interactuar fuertemente con proteoglucanos no agregantes de alto peso molecular. El inmunoblotting para la fibromodulina fue positivo para estas moléculas. No se observaron diferencias en cuanto a los aspectos cuantitativos, pero el tratamiento con anti-fibromodulina también fue positivo para moléculas con 150 kDa. Estas moléculas desaparecen cuando la muestra es tratada con agentes reductores. Este fenómeno puede estar relacionado con la autoagregación. La presencia de FM agregado de 150 kDa fue más prominente en el cartílago articular de la tibia. Estos resultados indican el efecto de la estimulación mecánica en las diferentes regiones de una misma articulación. Al considerar la relación existente entre la composición de la matriz y los estímulos mecánicos o químicos, se debe tener en cuenta que los condrocitos son sensibles a diferentes estímulo y pueden presentar un comportamiento metabólico heterogéneo en diferentes regiones de una misma articulación.

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Biografía del autor/a

Marcelo Augusto Marretto Esquisatto, Centro Universitário da Fundação Hermínio Ometto FHO

Biomédico, Mestre e Doutor em Biologia Celular e Estrutural.

Citas

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Publicado

2023-09-11

Cómo citar

Esquisatto, M. A. M., Erbert Contriciani, R., Rosa Pimentel, E., & Gomes, L. (2023). Población de proteoglicanos en diferentes sitios anatómicos de la cartílaga articular de la rodilla bovina. Revista Ensaios Pioneiros, 7(1). https://doi.org/10.24933/rep.v7i1.301

Número

Vol. 7 Núm. 1 (2023)

Sección

CIÊNCIAS BIOLÓGICAS E DA SAÚDE

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