POPULATION OF PROTEOGLYCANS IN DIFFERENT ANATOMICAL SITES OF BOVINE KNEE JOINT ARTICULAR CARTILAGE

Authors

  • Marcelo Augusto Marretto Esquisatto Centro Universitário da Fundação Hermínio Ometto FHO https://orcid.org/0000-0001-9786-6107
  • Renata Erbert Contriciani Centro Universitário da Fundação Hermínio Ometto FHO
  • Edson Rosa Pimentel Centro Universitário da Fundação Hermínio Ometto FHO
  • Laurecir Gomes Centro Universitário da Fundação Hermínio Ometto FHO

DOI:

https://doi.org/10.24933/rep.v7i1.301

Keywords:

Biomechanical forces, glycosaminoglycans, anatomical variation

Abstract

The mechanical forces that act on a synovial joint induce changes in the composition and organization of articular cartilage. Chondrocytes respond to stimuli both in a quantitative and qualitative manner with respect to the extracellular matrix. In the present study, it is investigated five different regions of the bovine knee joint in terms of proteoglycan populations and their glycosaminoglycan content. The high density PG population was isolated using ultracentrifugation (D1 fraction) and gel filtration. The PG presented similar size in all regions, but in tibia these molecules present higher Mr. The chondroitin sulfate was the more prominent glycosaminoglycan detected from D1 fraction and their chains presented around 40 KDa.  Two populations of non-aggregating PG were isolated in similar quantity from all regions These molecules presented polydisperse characteristics and migrating at about 70 and 200 kDa and exhibiting a behavior similar to that of the small proteoglycans decorin and biglycan. The presence of decorin was confirmed by immunoblotting. Agarose gel analysis after papain digestion showed the presence of dermatan sulfate in these PGs. The presence of decorin and especially of biglycan in the D2 fraction suggests that these small proteoglycans can strongly interact with high molecular weight non-aggregating proteoglycans. Another PG population, with MW around 60 Kda, was also isolated in low density ultracentrifugation fractions (D4). The immunoblotting for fibromodulin was positive to these molecules. No difference was verified in relation to quantitative aspects, but the treatment to fibromodulin antibody was also positive to molecules with 150 kDa. These molecules desappear when the sample is treated with reduced agents. This phenomenon can be related to a self-aggregation.  The presence of 150 kDa aggregated FM was more prominent in the tibia articular cartilage. These results indicate the effect of mechanical stimulation on the different regions of the same joint. When considering the relationship existing between matrix composition and mechanical or chemical stimuli, one should keep in mind that chondrocytes are sensitive to different stimuli and may present a heterogeneous metabolic behavior in different regions of the same joint.

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Author Biography

Marcelo Augusto Marretto Esquisatto, Centro Universitário da Fundação Hermínio Ometto FHO

Biomédico, Mestre e Doutor em Biologia Celular e Estrutural.

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Published

2023-09-11

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Esquisatto, M. A. M., Erbert Contriciani, R., Rosa Pimentel, E., & Gomes, L. (2023). POPULATION OF PROTEOGLYCANS IN DIFFERENT ANATOMICAL SITES OF BOVINE KNEE JOINT ARTICULAR CARTILAGE. Revista Ensaios Pioneiros, 7(1). https://doi.org/10.24933/rep.v7i1.301

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Vol. 7 No. 1 (2023)

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BIOLOGY AND HEALTH SCIENCES

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