Restrição nutricional gestacional altera a organização da glândula adrenal em ratas

Autores

  • Bruno dos Santos Telles Centro Universitário da Fundação Hermínio Ometto FHO
  • Hércules Jonas Rebelato Centro Universitário da Fundação Hermínio Ometto FHO
  • Marcelo Augusto Marretto Esquisatto Centro Universitário da Fundação Hermínio Ometto FHO https://orcid.org/0000-0001-9786-6107
  • Rosana Catisti Centro Universitário da Fundação Hermínio Ometto FHO

DOI:

https://doi.org/10.24933/rep.v7i1.296

Palavras-chave:

Restrição calórica, Restrição proteica, Gravidez, Adrenal, Receptores de glicocorticóides/mineralocorticóides

Resumo

A má nutrição durante a gravidez causa adaptação metabólica e/ou estrutural permanente na prole. A glândula adrenal produz vários hormônios esteróides durante a gravidez. Assim, este estudo teve como objetivo avaliar a influência da dieta durante a gestação sobre as glândulas adrenais de ratas Wistar. Para isso, ratas Wistar prenhes de 10 semanas (p, n=15) e ratas não prenhes (np, n=15) foram divididas em três grupos e receberam dieta controle normoproteica (C, 17% de caseína, n=5), dieta hipocalórica isocalórica (PR, 6% de caseína, n=5) e 50% de restrição calórica (RC, 50% da dieta consumida pelo grupo C), durante um período de 21 dias. No 21º dia de gestação (grupos 21dG, p) ou no 21º dia de dieta (grupos np), após aprofundamento anestésico, a glândula adrenal direita foi coletada, pesada (massa total) e preparada para inclusão em Paraplast® para exame histomorfométrico e análise imuno-histoquímica (Ki-67, receptores de glicocorticóides (GR) e receptor de mineralocorticóide (MR)) nas diferentes áreas da glândula. Os dados, expressos como média ± DP, foram avaliados por análise de variância unidirecional com pós-teste de Tukey (p < 0,05). CR na gravidez aumentou a quantidade de receptores GR, MR e Ki-67 na glândula adrenal. O grupo npRC apresentou maior reatividade de GR em comparação com os animais que receberam uma dieta normal. A restrição proteica na gravidez diminui a RM adrenal. Os resultados permitiram concluir que, mesmo sem alterar o peso das glândulas adrenais, o grupo pRC foi o que mais sofreu estresse durante o estudo, sugerindo que a RC associada à gravidez pode causar alterações morfofuncionais nas glândulas adrenais.

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Biografia do Autor

Marcelo Augusto Marretto Esquisatto, Centro Universitário da Fundação Hermínio Ometto FHO

Biomédico, Mestre e Doutor em Biologia Celular e Estrutural.

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Publicado

2023-09-11

Como Citar

dos Santos Telles, B., Rebelato, H. J., Esquisatto, M. A. M., & Catisti, R. (2023). Restrição nutricional gestacional altera a organização da glândula adrenal em ratas. Revista Ensaios Pioneiros, 7(1). https://doi.org/10.24933/rep.v7i1.296

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v. 7 n. 1 (2023)

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CIÊNCIAS BIOLÓGICAS E DA SAÚDE

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